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BSC MythBusters - Can overcrowding a BSC lead to loss of protection?

Work conducted in Biosafety Cabinets (BSCs) is a common sight in many laboratories worldwide, but some misconceptions about their capabilities sometimes arise.

A BSC used for storage of materials or the air grilles or grates blocked is often observed, but no conclusive evidence has shown whether this will affect the BSCs performance.

Here we explore and test the effect of overcrowding a cabinet as well as blocking off the front grille to determine if it will maintain ISO Class 5 air cleanliness.